There are claims of synthesis of a room temperature superconductor. However, these claims have not been officially accepted by scientific communities. Currently, the highest transition temperature (Tc) recognized in scientific articles is 135 K at 1 atm of Hg-Ba-Ca-Cu-O system which is a copper oxide superconductor. We packed graphite flakes into a ring-shaped polytetrafluoroethylene (PTFE) tube and further injected heptane or octane. Then we generated circulating current in this ring tube by electromagnetic induction and showed that this circulating current continues to flow continuously at room temperature for 50 days. This experiment suggests that bringing alkane into contact with graphite may result in a material with zero resistance at room temperature. In addition, we showed by means of AC resistance measurements using the two-terminal method that the resistances of graphite fibers brought into contact with various alkanes suddenly change at specific critical temperatures between 363 and 504 K. In this study, we show that after a magnetic field is applied to a single-layer graphene at room temperature, alkane is brought into contact with the single-layer graphene, then the graphene excludes the magnetic field immediately. This phenomenon demonstrates that the alkane-wetted single-layer graphene shows Meissner effect at room temperature.